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Already since the mid-nineteenth century, many observations have indicated that cell death plays
a considerable role during physiological processes of multicellular organisms, particularly during
embryogenesis and metamorphosis [Gluecksmann, 1951; Lockshin, 2001]. The term programmed cell death
was introduced in 1964, proposing that cell death during development is not of accidential nature but
follows a sequence of controlled steps leading to locally and temporally defined self-destruction [Lockshin, 1964].
Eventually, the term apoptosis had been coined in order to describe the morphological processes
leading to controlled cellular self-destruction and was first introduced in a publication by Kerr,
Wyllie and Currie [Kerr, 1972]. Apoptosis is of greek origin, having the meaning "falling off or dropping off",
in analogy to leaves falling off trees or petals dropping off flowers. This analogy emphasizes that the death of
living matter is an integral and necessary part of the life cycle of organisms. The apoptotic mode of cell death
is an active and defined process which plays an important role in the development of multicellular organisms and
in the regulation and maintenance of the cell populations in tissues upon physiological and pathological conditions.
It should be stressed that apoptosis is a well-defined and possibly the most frequent form of programmed cell death,
but that other, non-apoptotic types of cell death also might be of biological significance [Leist, 2001a].
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The development and maintenance of multicellular biological systems depends on a sophisticated interplay between the cells forming the organism,
it sometimes even seems to involve an altruistic behaviour of individual cells in favour of the organism as a whole. During development many cells
are produced in excess which eventually undergo programmed cell death and thereby contribute to sculpturing many organs and tissues
[see Figure 1 and Meier, 2000].
A particularly instructive example for the implication of programmed cell death in animal development is the formation of free and independent digits
by massive cell death in the interdigital mesenchymal tissue [Zuzarte-Luis, 2002]. Other examples are the development of the brain, during which half
of the neurons that are initially created will die in later stages when the adult brain is formed [Hutchins, 1998] and the development of the reproductive
organs [Meier, 2000]. Also cells of an adult organism constantly undergo physiological cell death which must be balanced with proliferation in order to
maintain homeostasis in terms of constant cell numbers. The majority of the developing lymphocytes die either during genetic rearrangement events in
the formation of the antigen receptor, during negative selection or in the periphery, thereby tightly controlling the pool of highly efficient and functional
but not self-reactive immune cells and at the same time keeping lymphocyte numbers relatively constant [Rathmell, 2002].
Taken together, apoptotic processes are of widespread biological significance, being involved in e.g. development, differentiation, proliferation/homoeostasis,
regulation and function of the immune system and in the removal of defect and therefore harmful cells. Thus, dysfunction or dysregulation of the apoptotic
program is implicated in a variety of pathological conditions. Defects in apoptosis can result in cancer, autoimmune diseases and spreading of viral infections,
while neurodegenerative disorders, AIDS and ischaemic diseases are caused or enhanced by excessive apoptosis [Fadeel, 1999a].
Due to its importance in such various biological processes, programmed cell death is a widespread phenomenon, occuring in all kinds of metazoans [Tittel, 2000]
such as in mammals, insects [Richardson, 2002], nematodes [Liu, 1999b], and cnidaria [Cikala, 1999]. Moreover, programmed cell death also might play a role in
plant biology [Solomon, 1999], and apoptosis-like cell death mechanisms even have been observed and used as a model system in yeast
[Frohlich, 2000; Skulachev, 2002]. Fascinating insights into the origin and evolution of programmed cell death might possibly be given by the fact, that
programmed cell death is also an integral part of the life cycle of other unicellular eukaryotes (such as the kinetoplastid parasite Trypanosoma brucei brucei,
the ciliate Tetrahymena thermophila, and the slime mold Dictyostelium discoideum) and that even prokaryotes (such as Bacillus subtilis, Streptomyces and
Myxobacteria) sometimes undergo regulated cell death [Ameisen, 2002].
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Apoptotic cells can be recognized by stereotypical morphological changes: the cell shrinks, shows deformation and looses contact to its neighbouring cells.
Its chromatin condenses and marginates at the nuclear membrane, the plasma membrane is blebbing or budding, and finally the cell is fragmented into compact
membrane-enclosed structures, called 'apoptotic bodies' which contain cytosol, the condensed chromatin, and organelles (Figure 2). The apoptotic bodies are
engulfed by macrophages and thus are removed from the tissue without causing an inflammatory response. Those morphological changes are a consequence
of characteristic molecular and biochemical events occurring in an apoptotic cell, most notably the activation of proteolytic enzymes which eventually mediate
the cleavage of DNA into oligonucleosomal fragments as well as the cleavage of a multitude of specific protein substrates which usually determine the integrity
and shape of the cytoplasm or organelles [Saraste, 2000].
Apoptosis is in contrast to the necrotic mode of cell-death in which case the cells suffer a major insult,
resulting in a loss of membrane integrity, swelling and disrupture of the cells. During necrosis, the cellular contents are released uncontrolled into the cell's
environment which results in damage of surrounding cells and a strong inflammatory response in the corresponding tissue [Leist, 2001a].
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This paragraph is meant to provide a general overview of basic apoptotic signalling pathways and of the molecular machinery responsible
for the induction and execution of apoptosis. The most important signalling molecules and cellular structures will be discussed in context of
their function and of the mechanisms in which they are involved in the initiation, mediation, execution, and regulation of apoptosis. This chapter
should give an impression of the sophisticated interplay between factors that promote or suppress apoptosis, resulting in a complicated regulatory
network which determines the fate of an individual cell as part of its multicellular environment (Figure 6).
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Apoptosis is a tightly regulated and at the same time highly efficient cell death program which requires the interplay of a multitude of factors.
The components of the apoptotic signalling network are genetically encoded and are considered to be usually in place in a nucleated cell ready
to be activated by a death-inducing stimulus [Ishizaki, 1995; Weil, 1996].
Apoptosis can be triggered by various stimuli from outside or inside the cell, e.g. by ligation of cell surface receptors, by DNA damage as a
cause of defects in DNA repair mechanisms, treatment with cytotoxic drugs or irradiation, by a lack of survival signals, contradictory cell cycle
signalling or by developmental death signals. Death signals of such diverse origin nevertheless appear to eventually activate a common cell death
machinery leading to the characteristic features of apoptotic cell death.
Much of the understanding of cell death has come from genetic studies in the nematode C. elegans by which several genes have been identified that
function in the apoptotic killing and elimination of 131 of the initially 1090 somatic cells that are generated druing hermaphrodite development [Hengartner, 1999].
The proximal cause of apoptosis in C. elegans is the activation of the cysteine protease ced-3, which is mediated by its oligomerization at the activator protein ced-4.
Activity of the ced-3/ced-4 complex is regulated by the apoptosis inhibitor ced-9 and the apoptosis inducer egl-1 (Figure 3). Subsequent studies in mammals
and in the fly, D. melanogaster, have identidied counterparts for these C. elegans genes, demonstrating that the core components of the cell death machinery
are conserved through evolution [Richardson, 2002]. Accordingly, ced-3 is the single C. elegans member of a family of cysteine proteases, the caspases,
whereas ced-4 corresponds to the mammalian apoptotic protease activating factor 1, Apaf-1, which is the core of a caspase-activating signalling complex,
the apoptosome. Egl-1 and ced-9 are members of the Bcl-2 family of pro- or antiapoptotic proteins, respectively, which play an important role in the mediation
and regulation of apoptotic signalling pathways. All of those central components will be discussed within the following paragraphs.
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The caspases, cysteine proteases homologous to C. elegans ced-3, are of central importance in the apoptotic signalling network which are activated in
most cases of apoptotic cell death [Bratton, 2000]. Actually, strictly defined, cell death only can be classified to follow a classical apoptotic mode if
execution of cell death is dependent on caspase activity [Leist, 2001a].
The term caspases is derived from cysteine-dependent aspartate-specific proteases: their catalytical activity depends on a critical cysteine-residue
within a highly conserved active-site pentapeptide QACRG, and the caspases specifically cleave their substrates after Asp residues. So far, 7 different
caspases have been identified in Drosophila, and 14 different members of the caspase-family have been described in mammals, with caspase-11 and
caspase–12 only identified in the mouse [Denault, 2002; Richardson, 2002]. According to a unified nomenclature, the caspases are referred to in the
order of their publication: caspase-1 is ICE (Interleukin-1ß-Converting Enzyme), the first mammalian caspase described to be a homologue of Ced-3
[Creagh, 2001; Miura, 1993]. Caspase-1 as well as caspases-4, -5, -11, and –12 appear to be mainly involved in the proteolytic maturation of pro-inflammatory
cytokines such as pro-IL-1ß and pro-IL-18 and their contribution to the execution of apoptosis remains questionable [Denault, 2002]. Indeed, mice deficient for
caspase-1 or caspase-11 develop normally and cells from those knockout mice remain sensitive to various death stimuli [Li, 1995; Wang, 1998]. In contrast,
gene knockout experiments targeting caspase-3 and caspase-9 resulted in perinatal mortality as a result of severe defects in brain development [Kuida, 1998;
Kuida, 1996], whereas caspase-8 deficient embryos died after day 12 [Varfolomeev, 1998]. This and the observation that cell lines derived from those knockout
experiments are resistant to distinct apoptosis stimuli underlines the importance of caspases as proapoptotic mediators. Indeed, caspase-3, caspase-9, caspase-8,
and additionally caspases-2, -6, -7, and –10 have been recognized to play an important role in the apoptotic signalling machinery [Earnshaw, 1999].
In the cell, caspases are synthesized as inactive zymogens, the so called procaspases, which at their N-terminus carry a prodomain followed by a large and a
small subunit which sometimes are separated by a linker peptide. Upon maturation, the procaspases are proteolytically processed between the large and small
subunit, resulting in a small and a large subunit. The prodomain is also frequently but not necessarily removed during the activation process. A heterotetramer
consisting of each two small and two large subunits then forms an active caspase. The proapoptotic caspases can be divided into the group of initiator caspases
including procaspases-2, -8, -9 and –10, and into the group of executioner caspases including procaspases-3, -6, and –7. Whereas the executioner caspases
possess only short prodomains, the initiator caspases possess long prodomains, containing death effector domains (DED) in the case of procaspases-8 and –10 or
caspase recruitment domains (CARD) as in the case of procaspase-9 and procaspase-2.
Via their prodomains, the initiator caspases are recruited to and activated at death inducing signalling complexes either in response to the ligation of cell surface
death receptors (extrinsic apoptosis pathways) or in response to signals originating from inside the cell (intrinsic apoptosis pathways).
In extrinsic apoptosis pathways (Figure 4), e.g. procaspase-8 is recruited by its DEDs to the death inducing signalling complex (DISC), a
membrane receptor complex formed following to the ligation of a member of the tumor necrosis factor receptor (TNFR) family [Sartorius, 2001].
When bound to the DISC, several procaspase-8 molecules are in close proximity to each other and therefore are assumed to activate each other by
autoproteolysis [Denault, 2002].
Intrinsic apoptosis pathways (Figure 5) involve procaspase-9 which is activated downstream of mitochondrial proapoptotic events at the so
called apoptosome, a cytosolic death signalling protein complex that is formed upon release of cytochrome c from the mitochondria [Salvesen, 2002b].
In this case it is the dimerization of procaspase-9 molecules at the Apaf-1 scaffold that is responsible for caspase-9 activation [Denault, 2002].
Once the initiator caspases have been activated, they can proteolytically activate the effector procaspases-3, -6, and -7 which subsequently cleave a specific
set of protein substrates, including procaspases themselves, resulting in the mediation and amplification of the death signal and eventually in the execution of
cell death with all the morphological and biochemical features usually observed [Earnshaw, 1999].
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Extrinsic apoptosis signalling is mediated by the activation of so called “death receptors” which are cell surface receptors that transmit apoptotic
signals after ligation with specific ligands. Death receptors belong to the tumor necrosis factor receptor (TNFR) gene superfamily, including TNFR-1,
Fas/CD95, and the TRAIL receptors DR-4 and DR-5 [Ashkenazi, 2002]. All members of the TNFR family consist of cysteine rich extracellular
subdomains which allow them to recognize their ligands with specificity, resulting in the trimerization and activation of the respective death receptor
[Naismith, 1998]. Subsequent signalling is mediated by the cytoplasmic part of the death receptor which contains a conserved sequence termed the
death domain (DD). Adapter molecules like FADD or TRADD themselves possess their own DDs by which they are recruited to the DDs of the
activated death receptor, thereby forming the so-called death inducing signalling complex (DISC). In addition to its DD, the adaptor FADD also
contains a death effector domain (DED) which through homotypic DED-DED interaction sequesters procaspase-8 to the DISC (Figure 4).
As described above, the local concentration of several procaspase-8 molecules at the DISC leads to their autocatalytic activation and
release of active caspase-8. Active caspase-8 then processes downstream effector caspases which subsequently cleave specific substrates resulting in
cell death. Cells harboring the capacity to induce such direct and mainly caspase-dependent apoptosis pathways were classified to belong to the so called
type I cells [Scaffidi, 1998].
In type II cells, the signal coming from the activated receptor does not generate a caspase signalling cascade strong enough for execution of cell death on its own.
In this case, the signal needs to be amplified via mitochondria-dependent apoptotic pathways. The link between the caspase signalling cascade and the
mitochondria is provided by the Bcl-2 family member Bid. Bid is cleaved by caspase-8 and in its truncated form (tBID) translocates to the mitochondria
where it acts in concert with the proapoptotic Bcl-2 family members Bax and Bak to induce the release of cytochrome c and other mitochondrial proapoptotic
factors into the cytosol (see Figure 6 and [Luo, 1998]). Cytosolic cytochrome c is binding to monomeric Apaf-1 which then, in a dATP-dependent conformational
change, oligomerizes to assemble the apoptosome, a complex of wheel-like structure with 7-fold symmetry, that triggers the activation of the initiator procaspase-9
(see Figure 5 and [Acehan, 2002]). Activated caspase-9 subsequently initiates a caspase cascade involving downstream effector caspases such as caspase-3,
caspase-7, and caspase-6, ultimately resulting in cell death [Slee, 1999].
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Besides amplifying and mediating extrinsic apoptotic pathways, mitochondria also play a central role in the integration and propagation of death signals
originating from inside the cell such as DNA damage, oxidative stress, starvation, as well as those induced by chemotherapeutic drugs [Kaufmann, 2000; Wang, 2001].
Most apoptosis-inducing conditions involve the disruption of the mitochondrial inner transmembrane potential (Dy) as well as the so called permeability transition (PT),
a sudden increase of the inner mitochondrial membrane permeability to solutes with a molecular mass below approximately 1.5 kDa. Concomitantly, osmotic
mitochondrial swelling has been observed by influx of water into the matrix with eventual rupture of the outer mitochondrial membrane, resulting in the
release of proapoptotic proteins from the mitochondrial intermembrane space into the cytoplasm [Bernardi, 1999; Loeffler, 2000]. Released proteins include
cytochrome c, which activates the apoptosome and therefore the caspase cascade, but also other factors such as the apoptosis-inducing factor AIF [Susin, 1999],
the endonuclease endoG [Li, 2001], Smac/Diablo [Verhagen, 2000], and Htr/Omi [Verhagen, 2002]. Interestingly, PT is always followed by Dy, but Dy is not
always caused by PT, and cytochrome c release has been observed even in absence of Dy [Bernardi, 1999; Kroemer, 2000]. In addition to the release of
mitochondrial factors, the dissipation of Dy and PT also cause a loss of the biochemical homeostasis of the cell: ATP synthesis is stopped, redox molecules
such as NADH, NADPH, and glutathione are oxidized, and reactive oxygen species (ROS) are increasingly generated [Kroemer, 2000; Kroemer, 1997].
Increased levels of ROS directly cause the oxidation of lipids, proteins, and nucleic acids, thereby enhancing the disruption of Dy as part of a positive feedback
[Marchetti, 1997]. Several possible mechanisms for PT have been proposed, but there appears to exist consent that a so-called permeability transition pore (PTP)
is formed consisting of the adenin nucleotide translocator (ANT) and the voltage-dependent anion channel (VDAC) as its core components. ANT is the most
abundant protein of the inner mitochondrial membrane and as a transmembrane channel is responsible for the export of ATP in exchange with ADP (antiport).
Overexpression of ANT-1 in human cancer cell lines dominantly induces apoptosis with all its characteristic features whereas its closely conserved homologue
ANT-2 does not, indicating a specific mechanistic role of ANT-1 in mitochondrial apoptosis events [Bauer, 1999]. VDAC, also called porin, is the most abundant
protein of the outer mitochondrial membrane and forms a non-selective pore through the outer membrane. Indicated by direct protein-protein interactions,
VDAC-ANT complexes presumably connect inner and outer mitochondrial membrane to so-called ‘contact sites’, corresponding to a close association of the
two membranes and thereby possibly constituting the PT pore [Beutner, 1998]. Since PT, loss of Dy, and release of mitochondrial proteins are of central
importance in mediating and enhancing apoptotic pathways, those mitochondrial events must be kept under strict control of regulatory mechanisms which are in
many ways dependent on members of the Bcl-2 family which will be discussed in the next paragraph.
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Commonly, the activation of apoptosis is regarded to occur when a cell encounters a specific death-inducing signal
such as the ligation of a death receptor by its cognate ligand or if cells are treated with a cytotoxic drug. This
suggests that the apoptosis signalling pathways in viable cells are kept in an inactive state and are only turned
on in response to a death stimulus. But it should be taken into account that the components of the apoptotic signalling
network are genetically encoded and ready for action in most cell types. Therefore, an interesting and possibly more
realistic alternative view would be as follows: all cells of a multicellular animal might be intrinsically programmed to
self-destruct and indeed would die instantaneously unless cell death is continously repressed by survival signals such
as provided by other cells of the organism, e.g. growth factors, hormones, nutrients. Those survival signals enhance
the expression and/or activity of antiapoptotic regulatory molecules thereby keeping in check the activation of proapoptotic
factors [Ameisen, 2002; Raff, 1993]. Indeed, a set of various antiapoptotic molecules and mechanisms has been
identified, as well as proapoptotic factors that counteract those inhibitory molecules when apoptotic demise of a cell is
timely and imperative.
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Bcl-2, an oncogene which in follicular lymphoma is frequently linked to an immunoglobulin locus by the chromosome translocation t(14:18),
was the first example of an oncogene that inhibits cell death rather than promoting proliferation. B cells transfected with Bcl-2 were shown
to be rendered resistant towards apoptosis induced by IL-3 withdrawal: for the first time it was shown that the pathway toward tumorigenesis
depends not only on the ability to escape growth control but also depends on the ability to prevent apoptosis [Vaux, 1988].
When homologues of Bcl-2 had been identified, it became apparent that a Bcl-2 family of proteins can be defined by the
presence of conserved sequence motifs known as Bcl-2 homology domains (BH1 to BH4). In mammals, up to 30 relatives
have been decribed of which some belong to a group of pro-survival members and others to a group of proapoptotic members [Borner, 2003].
In addition to Bcl-2 itself, there are a number of other prosurvival proteins, e.g. Bcl-XL, Bcl-w, A1, and Mcl-1, which all possess the domains
BH1, BH2, BH3, and BH4. The proapoptotic group of Bcl-2 members can be devided into two subgroups: the Bax-subfamily consists of Bax,
Bak, and Bok that all possess the domains BH1, BH2, and BH3, whereas the BH3-only proteins (Bid, Bim, Bik, Bad, Bmf, Hrk, Noxa, Puma,
Blk, BNIP3, and Spike) have only the short BH3 motif, an interaction domain that is both necessary and sufficient for their killing action
[Cory, 2002; Mund, 2003].
There has been quite some debate about how the Bcl-2 family controls apoptosis: one model proposes that Bcl-2 members might directly
control caspase activation [Strasser, 2000], whereas another model claims that they mainly act by guarding mitochondrial integrity [Wang, 2001].
In support of the first model, the worm Bcl-2 orthologue ced-9 binds to the Apaf-1-like adaptor protein ced-4 and prevents it from activating the
caspase ced-3 unless the BH3-only protein Egl-1 displaces ced-4 as shown in Figure 3 [Conradt, 1998]. In contrast, the mammalian ced-4
homologue Apaf-1 obviously does not interact with Bcl-2-like proteins [Moriishi, 1999] but is activated by cytosolic cytochrome c (see Figure 5),
and it is the release of cytochrome c from the mitochondria that can be controlled by Bcl-2 [Kluck, 1997; Yang, 1997]. Therefore it appears likely,
that the central function of mammalian Bcl-2 family members is to guard mitochondrial integrity and to control the release of mitochondrial proteins
into the cytoplasm [Cory, 2002].
How then is mitochondrial integrity affected by proapoptotic Bcl-2 family members? Central to this question are Bax and Bak, even though
inactivation of the Bax gene alone affected apoptosis only slightly and disruption of Bak alone did not show any effect. However, the double
knockout of Bax and Bak resulted in dramatic impairment of apoptosis during development in many tissues with superfluous cells accumulating
in the hematopoietic system and in the brain. Additionally, cells derived from those Bax -/- Bak -/- mice are insensitive to treatment with e.g.
etoposide or irradiation [Lindsten, 2000; Wei, 2001]. Bax is a cytosolic monomer in viable cells but during apoptosis changes its conformation,
integrates into the outer mitochondrial membrane and oligomerizes [Nechushtan, 2001]. Although the mechanism is controversial, Bax and Bak
oligomers are believed to provoke or contribute to the permeabilization of the outer mitochondrial membrane (PT), either by forming channels by
themselves [Antonsson, 2000] or by interacting with components of the PT pore such as VDAC [Tsujimoto, 2000].
In contrast, antiapoptotic Bcl-2 members sequester proapoptotic Bcl-2 members by bindig to their BH3 domains and thereby ultimately prevent
Bax or Bak activation/ oligomerization and consequently inhibit mitochondrial proapoptotic events: overexpression of Bcl-2 or Bcl-XL potently
inhibits apoptosis in response to many cytotoxic insults, among others by suppressing the generation of ROS, stabilizing Dy, preventing PT and
consequently blocking the release of e.g. cytochrome c [Reed, 1998]. Besides eliciting its antiapoptotic effects on the mitochondrial level by indirectly
controlling the activation of the apoptosome, Bcl-2 also appears to inhibit apoptotic pathways that are independent of Apaf-1/caspase-9 and which might
depend on caspase-7 as a central effector [Marsden, 2002]. In this context one might even expect the existence of another but up to now unidentified
Apaf-1 homologue that can be directly controlled by Bcl-2/Bcl-XL [Puthalakath, 2002].
Whereas Bax and Bak represent the central core of a proapoptotic Bcl-2 death machinery that is held in check by the pro-survival members Bcl-2
and Bcl-XL, members of the BH3-only subfamily are required for the activation of proapoptotic Bax/Bak function [Bouillet, 2002].
On the other hand, the killing effect of BH3-only members depends on Bax/Bak, since cells double-deficient for Bax and Bak do
not die upon overexpression of BH3-only proteins as it would be the case in wildtype cells, indicating that BH3-only members function
upstream of Bax and Bak [Lindsten, 2000; Wei, 2001]. Importantly, just as the proapoptotic activity of multidomain proteins Bax and
Bak is controlled by their interaction with the antiapoptotic guardians Bcl-2/Bcl-XL, also most BH3-only members display a strong
binding preference to antiapoptotic Bcl-2/Bcl-XL and in this way are kept under control [Scorrano, 2003].
Individual BH3-only proteins are believed to transduce specific death signals since they can be activated for apoptosis signalling
by sensing cell stress, such as DNA damage (Noxa and Puma are p53-inducible genes), growth factor deprivation (Hrk and Bim mRNA
expression is increased), or anoikis (Bmf is activated by subcellular relocalization) [Borner, 2003]. As another example, cytoplasmic
Bid is processed by caspase-8 to its truncated form tBid, which after myristoylation translocates to the mitochondria where it triggers
cytochrome c release by affecting Bax/Bak oligomerization and/or by mobilizing cytochrome c stores in cristae [Cory, 2002; Scorrano, 2002].
In general, BH3-only proteins are thought to interfere with the fine-tuned balance of homo- or heterooligomerization between proapoptotic
multidomain members Bax/Bak and antiapoptotic members Bcl-2/Bcl-XL (Figure 7). It has been proposed that Bid and Bim possess
BH3 domains (Bid-like BH3 domain) which can directly mediate Bax/Bak oligomerization, whereas Bad and Bik possess Bad-like BH3
domains which do not directly act on Bax/Bak but preferentially interact with antiapoptotic Bcl-2/Bcl-XL. As a consequence, activated
Bad/Bik might be able to displace Bid/Bim from the binding pocket of antiapoptotic Bcl-2/Bcl-XL, and - in this way released - Bid/Bim
might provoke Bax/Bak oligomerization and cytochrome c release even at subliminal levels [Letai, 2002].
In summary, a current model of how Bcl-2 family members regulate apoptosis can be descibed as follows (Figure 7): specific apoptotic
stress signals trigger the activation of particular BH3-only proteins which then interact with antiapoptotic members on the outer mitochondrial
(but also nuclear/ER) membrane, resulting in the release of Bax-like proapoptotic factors. Bax-like factors undergo a conformational change
(possibly assisted by some BH3-only proteins), insert into the outer mitochondrial membrane where they provoke PT and the release of
apoptogenic factors [Borner, 2003]
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Expression levels of antiapoptotic proteins such as Bcl-2, Bcl-XL, and A1 were reported to be upregulated by the transcription factor NF-kB
which besides being a central regulator of the innate and adaptive immune response is commonly described as an antiapoptotic transcription
factor [Heckman, 2002; Karin, 2002], although under certain circumstances NF-kB also might positively contribute to apoptosis induction
[Grimm, 1996b]. Besides inducing the expression of pro-survival Bcl-2 members, NF-kB additionally transactivates a number of other
antiapoptotic genes, such as the IAPs (inhibitors of apoptosis proteins).
IAPs are a family of antiapoptotic proteins whose prototype originally was described in baculovirus with many homologues found to be
conserved across several species. So far, eight human IAP homologues have been identified, among others NAIP, c-IAP1, c-IAP2, XIAP
and survivin. All IAPs contain baculovirus IAP repeat (BIR) domains, 70 amino acid motifs, which are essential for the antiapoptotic properties of
IAPs [Takahashi, 1998] because it is the interaction between the BIR domains and caspases that is believed to confer most of the antiapoptotic activity of IAPs. Indeed, XIAP, c-IAP1 and c-IAP2 are thought to directly inhibit caspases-3, -7, and –9 [Salvesen, 2002a]. In case of XIAP, it is the BIR3 domain that directly binds to the small subunit of caspase-9, whereas it is the BIR2 domain that interacts with the active-site substrate binding pocket of caspases-3 and –7 [Huang, 2001; Srinivasula, 2001].
In addition to the BIR domains, c-IAP1, c-IAP2, and XIAP contain a highly conserved RING domain at their C-terminal end which possesses
E3 ubiquitin ligase activity. Via this RING domain, IAPs are able to catalyze their own ubiquitination, thereby targeting
themselves for degradation by the proteasome [Yang, 2000], but they also might target other proteins such as caspase-3 and –7 for ubiquitination
and degradation [Huang, 2000; Suzuki, 2001]. Direct inhibition of caspase activity by c-IAPs is certainly a very important means of regulation
when considered that signalling cascades mediated by proteolytic enzymes such as caspases is irreversible once activated and therefore must
be precisely regulated in order to prevent locally and temporally inappropriate demise of cells. Importantly, Smac/Diablo, when released from
the mitochondrial intermembrane space during mitochondrial apoptotic events, is able to counteract the inhibitory effect of IAPs on
caspases since Smac/Diablo can bind to e.g. XIAP in a manner that displaces caspases from XIAP and enables their activation.
Thus, Smac/Diablo is a negative regulator of IAPs and in this way unfolds its apoptosis-enhancing property [Du, 2000].
Essential for the ability of Smac/Diablo to bind to XIAP and to release caspases is a conserved tetrapeptide motif which is also
present in HtrA2/Omi, another mitochondrial proapoptotic factor [van Loo, 2002], as well as in the Drosophila proapoptotic proteins
Reaper, Hid, Grim, and Sickle which can bind to DIAP1, a Drosophila IAP and thereby support activation of the fly caspase Dronc [Hawkins, 2000].
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In the adult human body several hundred thousand cells are produced every second by mitosis, and a similar number die by apoptosis
for the maintenance of homeostasis and for specific tasks such as the regulation of immune cell selection and activity [Fadeel, 1999b].
Dysregulation of apoptotic signalling can play a primary or secondary role in various diseases with insufficient apoptosis leading to e.g.
cancer (cell acumulation, resistance to therapy, defective tumor surveillance by the immune system), autoimmunity (failure to eliminate
autoreactive lymphocytes), persistent infections (failure to eradicate infected cells), whereas excessive apoptosis contributes to e.g.
neurodegeneration (Alzheimers’ disease, Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis), autoimmunity
(uncontrolled apoptosis induction in specific organs), AIDS (depletion of T lymphocytes), and ischaemia (stroke, myocardial infarction)
[Reed, 2002]. Malfunction of the death machinery results from the mutation of genes that code for factors directly or indirectly involved
in the initiation, mediation, or execution of apoptosis, and several mutations in apoptosis genes have been identified as a causing or contributing
factor in human diseases [Mullauer, 2001].
Of special interest is the involvement of defective apoptosis pathways in tumor formation, progression, and metastasis as well as the occurrence
of multidrug resistance during cancer therapy [Johnstone, 2002]. During the last years it became more and more evident that tumorigenesis is not
merely the result of excessive proliferation due to the activation of oncogenes but to the same extent depends on the – frequently concurrent -
impairment of apoptosis checkpoints [Hanahan, 2000; Wang, 1999]. Intriguingly, many of the alterations that induce malignant transformation,
such as oncogene-driven deregulated proliferation and invasion, actually sensitize a cell to apoptosis, and therefore only those oncogenic
transformed cells will survive and become malignant which additionally acquire defects in apoptosis pathways and therefore are protected
against cell death induction [Vousden, 2002]. A transformed cell can achieve protection against apoptosis by inappropriate activation or
expression of antiapoptotic proteins (which usually act as oncogenes), or by the inactivation of proapoptotic factors (which usually are tumor-suppressors).
As an example and as already mentioned, Bcl-2 was the first apoptosis-related gene that was recognized to play a
role in tumorigenesis, and indeed, Bcl-2 is overexpressed in a variety of cancers, contributing to cancer cell survival through direct inhibition of
apoptosis [Hockenbery, 1990; Reed, 1999]. Conversely, mutated or downregulated Bax and Bak are observed in certain cancers
[Kondo, 2000; Rampino, 1997] and disruption of those genes promotes tumorigenesis in mice [Yin, 1997].
Further, proapoptotic Bad and procaspase-9 are negatively regulated by the oncogenic Akt/PKB kinase, which on the other hand is frequently
constitutively active or amplified in many types of human cancer [Nicholson, 2002], and its antagonist, the phosphatase PTEN, is one of the most
commonly mutated tumor-suppressors [Yamada, 2001]. Even more underlining its potential as an oncogene, Akt/PKB is also stimulating the
NF-kB survival pathway by phosphorylation of IkB kinase alpha (IKK alpha) and it is suppressing p53 proapoptotic signalling by phosphorylation of the
oncogene Mdm2 which thereby is activated for inhibition of p53 [Mayo, 2002]. Both, NF-kB and Mdm2, are themselves inappropriately activated or
overexpressed in the process of transformation [Chene, 2003; Orlowski, 2002].
A paradigm for the central importance of apoptosis checkpoints in the defense against malignant transformation presents the tumor suppressor p53,
which is presumably the most intensely studied apoptosis factor contributing to cancer because it is inactivated in presumably more than 50% of all
human cancers [Hainaut, 2000]. p53 is a tumor suppressor protein which is activated as a transcription factor in response to e.g. oncogene
activation, hypoxia and especially DNA damage, resulting in growth arrest and/or apoptosis by stimulating the expression of various p53 target
genes such as p21, Bax, Puma, Noxa, Apaf-1, Fas, and DR5 [Vousden, 2002] or by repressing the expression of antiapoptotic proteins, e.g.
Bcl-2, Bcl-XL or survivin [Hoffman, 2002; Wu, 2001]. Recent evidence suggests transcription-independent p53 apoptosis pathways in which p53
translocates to the mitochondria, interacts with Bcl-XL, induces PT and the release of cytochrome c [Mihara, 2003].
In non-stressed, undamaged cells p53 therefore must be kept under stringent control: it is present only at low cellular concentrations, it is
retained in the cytosol and prevented to enter the nucleus, and its transactivation domain is inactivated [Chene, 2003]. Central to p53 regulation
is the oncogene Mdm2 which binds to and thereby inhibits p53 (see Figure 8). Mdm2 is a ubiquitin-ligase which mediates ubiquitination of p53,
thereby targeting it for degradation by the proteasome. In this way, p53 levels are kept low in normal cells [Kubbutat, 1997]. The importance of
Mdm2 in the control of p53 is demonstrated by mdm2 gene knockout mice which die early during development but are rescued from death by
additional deletion of the p53 gene [Montes de Oca Luna, 1995].
In response to cellular stress (such as DNA damage), p53 is phosphorylated at specific serine/threonine residues which prevents the Mdm2-p53
interaction, and thus p53 is stabilized and activated [Schon, 2002]. Moreover, p53 is central to oncogene-induced cell death because it is induced
by oncogenes such as c-myc, adenovirus E1A, and ras as well as by loss of the retinoblastoma tumor suppressor pRb [Henriksson, 2001].
All those oncogenes activate the transcription factor E2F-1 which not only can promote cell cycle progression and proliferation but at the
same time directly triggers expression of the tumor suppressor ARF which leads to stabilization and activation of p53 [Ginsberg, 2002].
This explains in part why oncogene activation not always leads to uncontrolled cell proliferation but under certain circumstances to the
stabilization of p53 and activation of cell death, provided that p53 signalling pathways are intact [Eischen, 1999].
: The p53 network – survival and cell death regulation
Click the picture for a larger view
Therefore in many instances, an oncogenic insult only results in increased proliferation and eventually malignant transformation if activators of
p53 (such as ARF, Chk2, or ATM), p53 itself (e.g. by Mdm2, the adenovirus E1B, papillomavirus E6, or SV40 large T antigen), or p53
downstream signalling components (p53 target genes) are inactivated (Figure 8).
On the other hand, p53-mediated apoptosis pathways can be suppressed by survival signals, such as growth factors binding to their cognate
growth factor receptors what eventually results in activation of the Akt kinase [Datta, 1999]. Akt kinase is known to mediate a number of
antiapoptotic mechanisms, such as the direct phosphorylation and inactivation of Bad and caspase-9, the activation of NF-kB antiapoptotic
signalling via phosphorylation of IkB, but also phoshorylation and activation of Mdm2 as an inhibitor of p53 [Mayo, 2002]. Besides
phosphorylation, ubiquitination and protein-protein interactions, p53 is also regulated by acetylation what affects its transcriptional activity,
as well as by sumoylation [Melchior, 2003; Appella, 2001].
Gaining insight into the mechanisms and alterations by which components of the apoptotic machinery contribute to pathogenic processes,
should allow the development of more effective, higher specific and therefore better-tolerable therapeutic approaches. Those may include
the targeted activation of proapoptotic tumor suppressors or alternatively the blockade of antiapoptotic oncogenes in the case of cancer,
whereas for the treatment of premature cell death during e.g. neurodegeneration the inhibition of proapoptotic key components such as the
caspases might be promising [Reed, 2002].
go to Index
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